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Progenitor and Stem Cells Therapy in Bone and Cartilage Tissue...
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Progenitor and Stem Cells Therapy in Bone and Cartilage Tissue Engineering

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Stem Cells".

Deadline for manuscript submissions: closed (30 May 2023) | Viewed by 3778

Special Issue Editors

Dr. Mitsuo Ochi

E-Mail Website
Guest Editor
Graduate School of Biomedical Sciences, Hiroshima University, Higashihiroshima, Japan
Interests: cartilage regeneration
Dr. Naosuke Kamei

E-Mail Website
Guest Editor
Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
Interests: cartilage regeneration

Special Issue Information

Dear Colleagues,

Bone and cartilage diseases, such as osteoporosis, fractures, and osteoarthritis, are on the rise as society ages, and these diseases not only impair patients' quality of life but also have social and economic consequences. Bone and cartilage are the tissues for which regenerative medicine using stem cell-based tissue engineering has been studied the longest, and some of them have been applied clinically. However, the mechanisms of bone and cartilage regeneration are largely unknown. In addition, there are problems, such as heterogeneity of somatic stem cell populations, and individual differences in cell function in therapeutic applications, and cell quality evaluation methods have not been established. Furthermore, the combination of various regenerative scaffold materials and other factors may lead to differences in therapeutic efficacy. In addition to somatic stem cells, research is also being conducted to apply ES cells and iPS cells to bone and cartilage regeneration, but there are issues in terms of safety and cost.

We are pleased to invite you to submit an article for publication in the Special Issue on "Progenitor and Stem Cells Therapy in Bone and Cartilage Tissue Engineering". This Special Issue aims to consolidate research on the mechanisms in bone and cartilage regeneration using various stem and progenitor cells, evaluation and improvement of stem cell function, development of unique cell delivery and material combination therapies, and safety of ES and iPS cells in bone and cartilage regeneration. This Special Issue also welcomes research on new therapeutic strategies, such as drug discovery-type therapies using stem cells.

In this Special Issue, original research articles and reviews are welcome. Research areas may include (but are not limited to) the following: 

  • Effects and mechanisms of bone and cartilage regeneration by stem/progenitor cell transplantation.
  • Functional and quality evaluation of stem/progenitor cells for bone and cartilage regeneration.
  • Combination of stem/progenitor cell transplantation with cell delivery systems and scaffold materials for bone and cartilage regeneration.
  • Efficacy and safety of ES cells and iPS cells for bone and cartilage regeneration.
  • Drug discovery related to stem/progenitor cells for bone and cartilage regeneration (stem cell-based drug discovery technologies and drug discovery targeting resident stem/progenitor cells). 

We look forward to receiving your contributions. 

Dr. Mitsuo Ochi
Dr. Naosuke Kamei
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • stem cell
  • progenitor cell
  • bone
  • cartilage
  • mechanism
  • delivery
  • scaffold
  • ES cell
  • iPS cell
  • drug

Published Papers (2 papers)

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Research

18 pages, 2448 KiB  
Article
Inverse Regulation of Cartilage Neogenesis at Physiologically Relevant Calcium Conditions by Human Articular Chondrocytes and Mesenchymal Stromal Cells
by Tim Hammersen, Justyna Buchert, Severin Zietzschmann, Solvig Diederichs and Wiltrud Richter
Cells 2023, 12(12), 1659; https://0-doi-org.brum.beds.ac.uk/10.3390/cells12121659 - 18 Jun 2023
Viewed by 1203
Abstract
Elaborate bioreactor cultivation or expensive growth factor supplementation can enhance extracellular matrix production in engineered neocartilage to provide sufficient mechanical resistance. We here investigated whether raising extracellular calcium levels in chondrogenic cultures to physiologically relevant levels would provide a simple and inexpensive alternative [...] Read more.
Elaborate bioreactor cultivation or expensive growth factor supplementation can enhance extracellular matrix production in engineered neocartilage to provide sufficient mechanical resistance. We here investigated whether raising extracellular calcium levels in chondrogenic cultures to physiologically relevant levels would provide a simple and inexpensive alternative to enhance cartilage neogenesis from human articular chondrocytes (AC) or bone marrow-derived mesenchymal stromal cells (BMSC). Interestingly, AC and BMSC-derived chondrocytes showed an opposite response to a calcium increase from 1.8 mM to 8 mM by which glycosaminoglycan (GAG) and collagen type II production were elevated during BMSC chondrogenesis but depressed in AC, leading to two-fold higher GAG/DNA values in BMSC-based neocartilage compared to the AC group. According to control treatments with Mg2+ or sucrose, these effects were specific for CaCl2 rather than divalent cations or osmolarity. Importantly, undesired pro-hypertrophic traits were not stimulated by calcium treatment. Specific induction of PTHrP mRNA and protein by 8.0mM calcium only in AC, along with negative effects of recombinant PTHrP1-34 on cartilage matrix production, suggested that the PTHrP pathway contributed to the detrimental effects in AC-based neocartilage. Altogether, raising extracellular calcium levels was discovered as a novel, simple and inexpensive stimulator for BMSC-based cartilage neogenesis without the need for special bioreactors, whereas such conditions should be avoided for AC. Full article
(This article belongs to the Special Issue Progenitor and Stem Cells Therapy in Bone and Cartilage Tissue Engineering)
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18 pages, 4949 KiB  
Article
Heat Shock Protein 27 Is Involved in the Bioactive Glass Induced Osteogenic Response of Human Mesenchymal Stem Cells
by Laura Hyväri, Sari Vanhatupa, Miina Ojansivu, Minna Kelloniemi, Toni-Karri Pakarinen, Leena Hupa and Susanna Miettinen
Cells 2023, 12(2), 224; https://0-doi-org.brum.beds.ac.uk/10.3390/cells12020224 - 5 Jan 2023
Cited by 2 | Viewed by 2122
Abstract
Bioactive glass (BaG) materials are increasingly used in clinics, but their regulatory mechanisms on osteogenic differentiation remain understudied. In this study, we elucidated the currently unknown role of the p38 MAPK downstream target heat shock protein 27 (HSP27), in the osteogenic commitment of [...] Read more.
Bioactive glass (BaG) materials are increasingly used in clinics, but their regulatory mechanisms on osteogenic differentiation remain understudied. In this study, we elucidated the currently unknown role of the p38 MAPK downstream target heat shock protein 27 (HSP27), in the osteogenic commitment of human mesenchymal stem cells (hMSCs), derived from adipose tissue (hASCs) and bone marrow (hBMSCs). Osteogenesis was induced with ionic extract of an experimental BaG in osteogenic medium (OM). Our results showed that BaG OM induced fast osteogenesis of hASCs and hBMSCs, demonstrated by enhanced alkaline phosphatase (ALP) activity, production of extracellular matrix protein collagen type I, and matrix mineralization. BaG OM stimulated early and transient activation of p38/HSP27 signaling by phosphorylation in hMSCs. Inhibition of HSP27 phosphorylation with SB202190 reduced the ALP activity, mineralization, and collagen type I production induced by BaG OM. Furthermore, the reduced pHSP27 protein by SB202190 corresponded to a reduced F-actin intensity of hMSCs. The phosphorylation of HSP27 allowed its co-localization with the cytoskeleton. In terminally differentiated cells, however, pHSP27 was found diffusely in the cytoplasm. This study provides the first evidence that HSP27 is involved in hMSC osteogenesis induced with the ionic dissolution products of BaG. Our results indicate that HSP27 phosphorylation plays a role in the osteogenic commitment of hMSCs, possibly through the interaction with the cytoskeleton. Full article
(This article belongs to the Special Issue Progenitor and Stem Cells Therapy in Bone and Cartilage Tissue Engineering)
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