Targeted Deletion of Thymosin Beta 4 in Hepatic Stellate Cells Ameliorates Liver Fibrosis in a Transgenic Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Generation of Tβ4-Floxed Mice
2.2. Animal Experiments
2.3. RNA Analysis
2.4. Western Blot Assay
2.5. Liver Histology and Immunohistochemistry
2.6. HSC Isolation and Culture
2.7. Adenoviral Transfection in Primary HSCs Isolated from Mice
2.8. Measurement of ALT and AST
2.9. Hydroxyproline Assay
2.10. Statistics
3. Results
3.1. Generation of Tβ4 Conditional Knockout Mice
3.2. HSC-Targeted Knockout of Tβ4 Prevents Liver Fibrosis in Mice
3.3. Blocking Tβ4 in HSCs Attenuates Bile Duct Ligation (BDL)-Caused Liver Fibrosis in Mice
3.4. Tβ4 Restoration Reverses the Effect of Tβ4 Depletion on Mitigating Liver Fibrosis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, J.; Lee, C.; Han, J.; Jeong, H.; Wang, S.; Choi, Y.H.; Jung, Y. Targeted Deletion of Thymosin Beta 4 in Hepatic Stellate Cells Ameliorates Liver Fibrosis in a Transgenic Mouse Model. Cells 2023, 12, 1658. https://0-doi-org.brum.beds.ac.uk/10.3390/cells12121658
Kim J, Lee C, Han J, Jeong H, Wang S, Choi YH, Jung Y. Targeted Deletion of Thymosin Beta 4 in Hepatic Stellate Cells Ameliorates Liver Fibrosis in a Transgenic Mouse Model. Cells. 2023; 12(12):1658. https://0-doi-org.brum.beds.ac.uk/10.3390/cells12121658
Chicago/Turabian StyleKim, Jieun, Chanbin Lee, Jinsol Han, Hayeong Jeong, Sihyung Wang, Yung Hyun Choi, and Youngmi Jung. 2023. "Targeted Deletion of Thymosin Beta 4 in Hepatic Stellate Cells Ameliorates Liver Fibrosis in a Transgenic Mouse Model" Cells 12, no. 12: 1658. https://0-doi-org.brum.beds.ac.uk/10.3390/cells12121658